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. 2018 Apr 30;9(1):66–72. doi: 10.1016/j.jtcme.2017.12.002

Fig. 5.

Fig. 5

Effects of CRE and CSE on expression of melanogenic proteins. B16F10 cells were treated with CRE and CSE in the presence of 1 μM α-MSH for 48 h. After treatment, total protein was extracted with RIPA buffer and levels of (A) MITF (C) tyrosinase (E) TRP-1 and (G) TRP-2 proteins were determined by western blot analysis, using GAPDH as a loading control. Results (B, D, F, H) from three independent experiments are expressed as mean ± SEM. ##P < .01, ###P < .001 vs control cells and **P < .01, ***P < .001 vs α-MSH group.