Figure 5.

Velocity of Migrating Clones during Ex Vivo Wound Healing Measured by Quantitative STICS Analysis

(A) Eyes from Confetti mice (n = 3/group) were enucleated immediately post wounding, placed in organ culture, and imaged by light-sheet microscopy every 2 hr for 48 hr. Vector flow maps represent cell migration and direction of clone movement from images taken at 8 hr and 36 hr. Insets show the multi-directional movement of groups of cells within a clone, depicted as colored arrows; associated with these images is a heatmap that acts as a speed gauge.

(B) STICS was applied on a time series of 2D maximum-intensity projections of 3D image stacks. The speed of peripherally located clones every 2 hr for a representative cornea is displayed by colored histogram (from light to dark blue). The inset is a schematic representation of the wound, with arrows indicating the direction of closure.

See also Video S3.