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Effect of VCO on inhibition of inflammatory cytokines (a) TNF-α, (b) IFN-γ (c) IL-6 (d) IL-8 (e) IL-5 in THP-1 cells was done by ELISA. THP-1 cells were incubated with LPS alone, LPS with test samples in media containing 2% FBS for 24 h at 37 °C. Each values represented Mean ± SE of two determinations. *p < 0.05 statistical comparison with cell control was performed by student t-test. #p < 0.05 statistical comparison with LPS was performed by student t-test (n = 2).
