Figure 3.

Dach1 Gene Deletion Reduces Mammary Gland Cellular Proliferation, Ductal Branching, and Myoepithelial/Stem Cells

(A) Cellular proliferation assessed by Ki-67 immunostaining with nuclei marked by DAPI staining (A).

(B) As above, but quantified as mean ± SEM for n = 3 separate mice in each group. Data are shown for Dach1^wt/wtROSA26^{CreERT2/mTmGfl} versus Dach1^fl/flROSA26^{CreERT2/mTmGfl} after tamoxifen treatment as shown in Figure 1A.

(C) Ductal branching analysis (shown as mean ± SEM for n = 3 mice). Representative examples are shown at high magnification in Figure S3.

(D) A representative fluorescence-activated cell sorting (FACS) analysis from the mammary epithelium showing the separation of mT from mG cells, the subsequent separation of Lin⁻ cells and the apportioning of CD24/CD29 status.

(E) The proportion of Lin⁻ cells (cells from mammary gland excluding vascular and hematopoietic cells) was determined by FACS analysis and quantified for n = 4 separate mice. The proportion of Lin⁻ cells was not significantly altered between genotypes.

(F) The percentage of Lin⁻CD24^medCD29^hi cells was determined upon FACS analysis for n = 4 separate mice in each group.

(G) Luminal epithelial cells are shown as Lin⁻CD24^hi CD29^lo with data as mean ± SEM for n = 4 separate mice.