Figure 4.

Dach1 Gene Deletion Reduces the Abundance of Mammary Gland Stem Cell and Ductal Cell Lineage Gene Targets

(A) Immunofluorescent double staining for cytokeratin 5 (CK5) and CK8 showed that Dach1 gene deletion decreases the relative amount of CK5-positive cells versus CK8-positive cells.

(B) Immunohistochemical staining for differentiation factors (Gata3, Pml, and Pygo2) in the mammary gland of Dach1^+/+ versus Dach1^−/− (Cre deletion mice) (Dach1^wt/wtROSA26^{CreERT2/mTmGfl} versus Dach1^fl/flROSA26^{CreERT2/mTmGfl}). The red arrowheads indicate basal epithelial cells.

(C) Quantitation of immunohistochemical staining shown as mean ± SEM for n = 3 separate mice.