Figure 5.

DACH1 Occupies the Promoter Regions of Mammary Gland Stem Cell and Ductal Cell Lineage Gene Targets

(A–F) qRT-PCR analysis of mRNA for genes participating in mammary gland stem cell and ductal development, (A) Dach1, (B) Gata3, (C) Elf5, (D)Pml, (E) Notch1, and (F) Pygo2, in the mammary gland of Cre deletion mice (Dach1^wt/wtROSA26^{CreERT2/mTmGfl} versus Dach1^fl/flROSA26^{CreERT2/mTmGfl}) with quantitation shown as mean ± SEM for n = 3 separate mice. Notch1 mRNA levels were modestly reduced in the Dach1^−/− mammary gland.

(G–L) Analysis of DACH1 binding to these target genes was assessed using chromatin immunoprecipitation sequencing assays conducted on DACH1 stable MDA-MB-231 cells (G). (H) Gata3, (I) Elf5, (J) Pml, (K) Notch1, and (L) Pygo2.