Table 1.
Quantitative reverse transcription PCR primer‐probe pairs used to detect hepatitis‐associated viruses in equine liver or serum samples
| Name | Specificity | Sequence | Source |
|---|---|---|---|
| TDAV‐UTR171F | TDAV forward | AGGGTTCTTCGGGTAAATCC | Chandriani et al5 |
| TDAV‐UTR336Rd | TDAV reverse | CCCTCGGACTGAATTrTAGGC | Modified from Chandriani et al5 |
| TDAV‐UTR274 | TDAV probe | ACCTCCCTCACGAAAGGTCGCCAC | Current study |
| QANTI‐5UF1 | NPHV forward | GAGGGAGCTGRAATTCGTGAA | Burbelo et al32 |
| QANTI‐5UR1 | NPHV reverse | GCAAGCATCCTATCAGACCGT | Burbelo et al32 |
| NPHV‐UTR288 | NPHV probe | CCACGAAGGAAGGCGGGGGC | Burbelo et al32 |
| EPgV‐80F | EPgV forward1 | ACCGAGCCGCCCTGTAG | Current study |
| EPgV‐163R | EPgV reverse1 | CCTGCCACCGCGATCA | Current study |
| EPgV‐UTR122 | EPgV probe1 | TCCTGGCACTGGCCCGAAGC | Current study |
| EPgV‐127F | EPgV forward2 | GCACTGGCCCGAAGCAT | Current study |
| EPgV‐210R | EPgV reverse2 | CTGCCCTAACACAATCACAACAC | Current study |
| EPgV‐UTR165 | EPgV probe2 | TTCTTCGGGTAAATCCCGGCCG | Current study |
| EqPV‐3218F | EqPV‐H forward | ATGCAGATGCTTTCCGACC | Current study |
| EqPV‐3386R | EqPV‐H reverse | GCCCCAGAAACATATGGAAA | Current study |
| EqPV‐3310 | EqPV‐H probe | ACCGTAGCGGATTCGGGATCTGC | Current study |
Abbreviations: EPgV, equine pegivirus; EqPV‐H, equine parvovirus‐hepatitis; NPHV, non‐primate hepacivirus; TDAV, Theiler's disease–associated virus.