Figure 6.

hESCs Can Generate Functional Cell Types following Nocodazole Treatment

(A) qRT-PCR analysis for SMC markers in DMSO and nocodazole-treated cells. Error bars represent ±SEM of three independent experiments. Ordinary one-way ANOVA test followed by Sidak's test for comparison of DMSO versus nocodazole-treated cells was performed. (ns, not significant).

(B) Contractility of SMCs was induced by carbachol. Panels show cells contracting within 10 min of carbachol treatment. Graph shows % contraction of 20 cells in DMSO control and nocodazole-treated cells. Error bars represent ±SEM.

(C) qRT-PCR analysis for cardiomyocyte markers in DMSO and nocodazole-treated cells. Error bars represent ±SEM of three independent experiments. Ordinary one-way ANOVA test followed by Sidak's test for comparison of DMSO versus nocodazole-treated cells was performed (ns, not significant).

(D) Graph showing beating rate of cardiomyocytes generated form DMSO and nocodazole-treated cells. Error bars represent ±SEM (n = 4).

(E) qRT-PCR analysis for chondrocyte markers in DMSO- and nocodazole-treated cells. Error bars represent ±SEM of two independent experiments.

(F) Alcian blue staining of chondrocytes shows Alcian blue absorption and release of DMSO control and nocodazole-treated cells. Error bars represent ±SEM of triplicates in an independent experiment.

(G) qRT-PCR analysis for hepatocyte markers in DMSO and nocodazole-treated cells. Error bars represent ±SEM of triplicates in an independent experiment.

(H) Hepatocytes generated from DMSO and nocodazole-treated cells display cytochrome P450 3A4 activity, as assessed by the enzymatic release of free luciferin by cytochrome P450 from an inactive luciferin precursor. Error bars represent ±SEM of triplicates in an independent experiment.