Figure 2.

Increased Network Activity in TSC Neuron Mono-cultures Can Be Modulated by Rapa

(A–D) Representative traces of calcium influx of neuronal cultures generated from control and TSC patients, under Std (A and C) and Std + Rapa (B and D) culture conditions. The multicolor traces in (A–D) represent activity in 12 regions of interest per FOV.

(E) Average calcium event frequency for the patient (P1 and P2) and control (C1, C2, and C3) neurons under Std and Std + Rapa (means ± SEM). Average for P1 was obtained from P1A and P1B. The average calcium event frequency of every iPSC line is calculated by averaging the analysis of two to four differentiations. Asterisks in (E) indicate statistical differences found in Tukey's post hoc test, ^∗p < 0.05.

(F and G) Calcium event amplitude (F) and event area (G) in control and patient groups under Std and Std + Rapa conditions.

(H–L) Average spike rate (Hz) at 50 days post plating on MEAs under Std and Std + Rapa condition from C1, C2, P1A, and P2 (H) (n = 6–8 independent experiments; means ± SEM). Asterisks in (H) indicate unpaired t test, ^∗p < 0.05. A representative raster plot showing the activity in control neurons under; (I) Std and (J) Std + Rapa conditions and TSC neurons under; (K) Std and (L) Std + Rapa conditions.

See also Figure S2.