Figure 3.

Cxcr4 Knockdown Results in Lineage Progression and a Shift Away from the Vasculature

(A) CXCR4 expression in FACS-sorted lineage cells from the V-SVZ. ^∗∗p < 0.01, ^∗∗∗p < 0.001.

(B) Percentage of EdU + TdTomato cells in V-SVZ whole mounts at 7, 21, and 35 days after tamoxifen injection (n = 4–5 mice/group; ^∗∗p < 0.01 by two-way analysis of variance).

(C) Confocal images of whole mounts immunostained for laminin to label vasculature and EdU to label actively proliferating cells at 7 and 35 days after recombination. Scale bar, 100 μm.

(D) Percentage of MASH1+ TdTomato cells in V-SVZ whole mounts at 7, 21, and 35 days after tamoxifen injection (n = 3–5 mice/group; ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001 by two-way analysis of variance).

(E) Numbers of TdTomato-positive neuroblasts did not significantly differ at any time point measured.

(F) Cxcr4 deletion significantly shifted distance of MASH1 relative to the vasculature (n = 3,642 cells across 4 Cxcr4^+/+ mice, n = 3,166 cells across 3 Cxcr4^fl/fl mice; p < 0.001 by Mann-Whitney test).

See also Figure S2.