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. 2018 Dec 13;12(1):152–164. doi: 10.1016/j.stemcr.2018.11.018

Figure 4.

Figure 4

Effect of Cltc Knockdown in mESCs Can Be Partially Rescued by Inhibiting TGF-β Signaling

(A) Bright-field micrographs showing AP staining and morphology of Cltc knockdown mESCs in the presence of TGF-β inhibitors RepSox or SB431542 (SB). Scale bar, 50 μm.

(B) Immunofluorescence micrographs showing OCT3/4 and E-CAD staining upon RepSox or SB431542 (SB) treatment in mESCs with Cltc knockdown. Scale bar, 50 μm.

(C) Bar graph showing the quantitation of OCT3/4 and E-CAD-positive mESC colonies upon RepSox or SB431542 (SB) treatment in Cltc knockdown mESCs. Error bars represent mean ± SD from three independent experiments (n = 3). ∗∗p < 0.01 by two-tailed Student’s t test. One-way ANOVA with post-hoc Tukey-Kramer test was used.