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. 2018 Sep 18;28(1):84–95. doi: 10.1093/hmg/ddy325

Figure 5.

Figure 5

Matrigel invasion assays show decreased invasion of an EVT cell line, HTR8-SVneo after ADAMTS12 or ADAMTS16 KD. (A) qRT-PCR of ADAMTS12 demonstrating KD efficiency of approximately 90% of ADAMTS12. (B) qRT-PCR of ADAMTS16 demonstrating KD efficiency of approximately 90% of ADAMTS16 mRNA. (C) Representative image of HTR8 cells treated with negative control siRNA construct after 24 h of invasion through a transwell insert coated with Matrigel. (D) Representative image of HTR8-SVneo cells treated with ADATMS12 siRNA shows a reduction in the number of cells that invade through a transwell insert coated with Matrigel after 24 h. (E) Representative image of HTR8-SVneo cells treated with ADAMTS16 siRNA shows a reduction in the number of cells that invade through a transwell insert coated with Matrigel after 24 h. (F) siRNA KD of ADAMTS12 (light grey bar) or ADAMTS16 (dark grey bar) in HTR8-SVneo invasion shows a significant decrease in invasion. Three independent experiments were carried out, and five representative images from each experiment were quantified. Fold change values for ADAMTS12 and ADAMTS16 were obtained through normalization to HPRT, a placental housekeeping gene, and then comparison to CTRL cells treated with a negative control siRNA construct. aP ≤ 0.01, bP ≤ 0.001, cP ≤ 0.0001. Data are expressed as mean ± SEM.