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. Author manuscript; available in PMC: 2019 Nov 16.
Published in final edited form as: Basic Res Cardiol. 2018 Nov 16;114(1):3. doi: 10.1007/s00395-018-0710-1

Fig. 6.

Fig. 6

Entinostat-CMC administration more efficiently attenuates myocardial fibrosis. a Schematic diagram detailing cardiac dissection for histopathology. Each heart was dissected into 5 × 3 mm blocks (B1-B5) prior to histopathological analysis. Planimetric measurements of b Masson’s trichrome-stained transverse myocardial tissue sections (B1-B5; 4 μm in depth) were used to enumerate scar size, reported in c mass (mg) and d percentage of LV (% scarmass/LVmass; % wt/wt) in vehicle- (n=11), DMSO CMC- (n=10), and entinostat CMC- (n=10) treated rat hearts. Dot plots report the arithmetic mean ± SEM. Scale bars=3 mm. e Myocardial collagen content was digitally enumerated via polarized light microscopy of picrosirius red stained mid-papillary level myocardial sections (top row: brightfield; bottom row: polarized light). Scale bars=1 mm. f Regional collagen content (risk and remote myocardial zones) was quantified in cardiac sections derived from vehicle- (n=11), DMSO CMC- (n=10), and entinostat CMC- (n=10) injected animals. Values are reported as regional collagen content (% area) ± SEM.