Table 1.
Effects of DE exposure on the serotonergic pathway of GD28 fetuses
| Control | Exposed | Effects of DE exposure | Effects of Sex | Exposure × Sex | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Male (n = 4) | Female (n = 4) | Male (n = 4) | Female (n = 4) | F (1,15) | p-value | F (1,15) | p-value | F (1,15) | p-value | |
| Trp (fmol/mg) | 77,941 ± 4357.6 | 77,737 ± 9898.7 | 66,086 ± 7164.3 | 79,000 ± 7635.3 | 0.139 | 0.716 | 0.983 | 0.343 | 0.582 | 0.462 |
| K (fmol/mg) | 14,844 ± 689.1 | 13,875 ± 1144.6 | 12,028 ± 1334.5 | 12,611 ± 1312.6 | 0.669 | 0.431 | 0.000 | 0.995 | 0.049 | 0.829 |
| 5-HT (fmol/mg) | 1718 ± 228.3 | 1546 ± 151.4 | 1081 ± 158.8 | 1187 ± 133.4 | 6.972 | 0.023* | 0.046 | 0.833 | 0.724 | 0.413 |
| 5-HT/Trp | 0.022 ± 0.004 | 0.020 ± 0.001 | 0.017 ± 0.003 | 0.015 ± 0.002 | 3.457 | 0.090 | 0.628 | 0.445 | 0.081 | 0.782 |
| 5-HIAA (fmol/mg) | 170.0 ± 39.16 | 163.8 ± 32.29 | 197.9 ± 73.28 | 156.4 ± 18.62 | 0.244 | 0.631 | 0.199 | 0.664 | 0.136 | 0.720 |
| 5-HIAA/5-HT | 0.098 ± 0.016 | 0.117 ± 0.011 | 0.184 ± 0.056 | 0.136 ± 0.023 | 0.822 | 0.384 | 0.455 | 0.514 | 0.638 | 0.441 |
Basal rates of Tryptophan (Trp), Kynurenine (K), Serotonin (5-HT) and 5-Hydroxyindoleacetic acid (5-HIAA), along with the correspondent ratios. Results were expressed as fentomoles/milligram of fresh tissues (fmol/mg). Results are shown as mean ± SEM. *p < 0.05 represented a significant difference between each group. 5-HT levels were decreased in exposed fetuses compared to controls. No significant difference existed between groups regarding the other variables of interest