Fig. 3.

Decreased glucose uptake caused by MOMIPP is not due to direct inhibition of Glut1 and is dependent on endosomal vacuolization. a [³H]2-DG uptake was assayed in mouse erythrocytes, in the presence of MOMIPP, phloretin + HgCl₂ or cytochalasin B, as described in the Methods. Assays were performed in triplicate. b Immunofluorescence localization of Glut1 was performed in U251 cells 4 h after addition of 10 μM MOMIPP or DMSO (control), as described in the methods. c U251 cells were treated with 10 μM MOMIPP, 100 nM Bafilomycin A1, or a combination of both compounds, and cell morphology was examined after 4 h. d [³H]2-DG uptake was measured in cells treated as in panel C. Each value is the mean (± SD) derived from three separate cultures. *p < 0.05 compared to control. N.S.; not significantly different from Bafilomycin A1 alone