Fig. 5.

Inhibition of JNK attenuates the cytotoxicity of methuosis-inducing IPPs. U251 cells were treated for 24 h with 10 μM MOMIPP, 2q, or an equivalent volume of DMSO (control), with (+) or without (−) the JNK inhibitor, SP600125 (75 μM). Aliquots of cell lysate containing equal amounts of protein were then subjected to immunoblot analysis for phospho- and total c-Jun (a),or phospho- and total Bcl-2 and Bcl-xL (b). The blots are representative of similar results obtained in three separate experiments. c Cell morphology was assessed by phase-contrast microscopy after a 24-h incubation with MOMIPP or 2q, in the presence or absence of SP600125. d U251 cells were incubated for 48 h with the indicated concentrations of MOMIPP, in the presence or absence of 75 μM SP600125, as indicated. CellTiterGlo® viability assays were performed at the 48 h endpoint