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. 2019 Jan 16;12:39. doi: 10.1186/s13071-018-3283-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Phylogenetic relationship between Culicoides species based on a 405 bp region of the cytochrome c oxidase subunit 1 gene. Culicoides sequences from individuals captured in Texas (GenBank: MH751225, MH751229-34, MH751236-42, MH751245, MH751247, MH751249-51, MH751253-57, MH751259-66, MH751268-72, MH751274-79, MH751281-82), Winsconsin, Wyoming and South Carolina (GenBank: MH751220, MH751222-24, MH751226-28, MH751235, MH751243, MH751244, MH751246, MH751248, MH751252, MH751258, MH751267, MH751273, MH751280) are listed and individually marked with a star to indicate sample for which morphological characterization and molecular sequence were performed. The bootstrap support values above < 90% are shown at the corresponding nodes. Additionally, publically available cox1 sequences from Texas were added to the phylogenetic tree (GenBank: KT794137.1; KT794138.1; KT794141.1; KT794142.1; KT794143.1; KT794144.1, KT794154.1; KT794155.1; KT794159.1; KT794161.1; KT794162.1; KT794164.1; KT794165.1; KT794167.1; KT7941371.1). The tree was rooted to the cox1 sequence of Atrichopogon levis (GenBank: KT092130.1)