Table 2.
Proteins identified by SDS-PAGE separation (pH3.0–10.0 gradient gels) followed by in-gel digestion and LC-MS analysis. Last column (Expression) represents the state of proteins upon PEO treatment. The applied PEO concentration was 150 µg mL−1.
| Spot No. | Protein name | Mascot Score | Molecular weight [kDa] | Peptides | UniProt/Accession. Number | Expression (rate of change) | Confirmation by RT-PCR (fold change) |
|---|---|---|---|---|---|---|---|
| 1 | Serine protease htrA | 555.0 | 50.9 | 15.0 | gi|218562840 | -3x | 0.47 |
| 2 | Major antigen cbf2/PEB4A | 612.5 | 30.4 | 23.0 | CBF2_CAMJE | -8x | 0.23 |
| 3 | Elongation factor Tu | 682.6 | 43.5 | 28.0 | EFTU_CAMJD | +4x | 2.82 |
| 4 | Uncharacterized protein | 463.7 | 20.9 | 15.0 | gi|121612795 | +6.5x | 5.75 |
| 5 | Major cell-binding factor PEB1 | 343.8 | 28.1 | 12.0 | PEB1A_CAMJE | +4x | 7.10 |
| 6 | Succinyl-coA synthetase alpha chain | 744.7 | 30.0 | 23.0 | gi|384447814 | +4x | 2.14 |
| 7 | Major antigenic peptide PEB3 | 137.2 | 27. 5 | 6.0 | gi|57237344 | +1.5x | 1.43 |
| 8 | Anti-oxidant AhpC/TSA family protein | 446.5 | 21.9 | 13.0 | gi|57237385 | +4x | 3.84 |
| 9 | Oxidoreductase subunit | 374.8 | 26.9 | 16.0 | gi|121613212 | +2x | 1.98 |
| 10 | Adenylate kinase OS | 337.5 | 21.3 | 11.0 | KAD_CAMJE | +3x | 2.28 |
| 11 | Probable thiol peroxidase OS | 702.7 | 18.4 | 23.0 | TPX_CAMJE | +3x | 2.56 |
| 12 | Molecular chaperone DnaK | 366.0 | 67.3 | 15.0 | gi|57237604 | +5x | 10.41 |