Table 1.
Identification of significantly changed fecal proteins from healthy dogs and dogs suffering from food responsive diarrhea (FRD).
| Spot IDa | Mr. (kDa) / pIb (±SD) |
Normalized quantity Healthy (x103)b |
Normalized quantity FRD (x103)b |
Protein namec | Score | Speciesc | Sequencesc | Mr. (kDa) / pIc | Accession number |
|---|---|---|---|---|---|---|---|---|---|
| A | 12.4±0.4/7.9±0.2 | 550±61 | 179±21 | Hemoglobin subunit beta | 16∗ | Bos Taurus | LLVVYPWTQR | 15.9/7.01 | gi|27819608 |
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| I | 14.6±0.9/8.7±0.3 | 161±23 | 422±102 | Hypothetical protein | 19∗ | Streptomyces sp. | PAAAAGTAVQ | 14.1/9.5 | WP_046507073.1 |
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| K | 17.3±0.7/ 9.5±0 | 359 ± 130 | 58± 21 | Putative Cytochrome P450 | § | Oryza sativa | RTLVVSTAAAAAD LYR | 54.8/ 9.1 | gi|50725157 |
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| K1 | 17.1±0.2/9.8±0 | 0 | 126 ± 113 | UDP-N-acetylglucosamine diphosphorylase | 18∗ | Zea mays | RIPSVHGYTSGLK | 20.5/8.5 | ONM18096.1 |
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| L | 22.3±0.8/5.1±0.2 | 0 | 386±79 | Isopentenyl-diphosphate delta-isomerase | 31∗ | Streptomyces sp. | QSGPRPFDPQEVA | 21.1/5.3 | WP_073935025.1 |
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| O1 | 15.0±0.7/4,7±0.2 | 0 | 118±17 | not found | - | ||||
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| O2 | 14.8±0.7/ 4.9±0.2 | 0 | 27.3±17.1 | Immunoglobulin J chain isoform 1 | § | Canis lupus familiaris | IIPSPDDPNE IVER | 18.1/4.7 | gi|57095596 |
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| O3 | 14.9±0.9/5.2±0.1 | 0 | 101±47 | not found | - | ||||
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| O4 | 13.7±0.6/5.6±0.1 | 0 | 113±32 | not found | - | ||||
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| S | 11.4±0.4/5.7±0.1 | 36±19 | 94±46 | DTW domain-containing protein | 19∗ | Microbulbifer donghaiensis | KTNTGALALAQCGNLVER | 17.4/5.7 | WP_073272148.1 |
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| T | 9.9±0.8/6.2±0.1 | 60±25 | 197±95 | hypothetical protein U973_01647 | 19∗ | Staphylococcus aureus | IAKGLETAINAINE | 10.6/5.8 | EZW68888.1 |
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| U | 44.8±1.3/5.3±0.2 | 148±41 | 528±155 | Coproporphyrinogen III oxidase | 33∗ | Pontibacillus chungwhensis | EPVHYKEEMEEQ | 44.6/5.6 | WP_036785728.1 |
aAssigned spot ID as indicated in Figure 1.
bExperimental values calculated from the 2-DE maps by the PDQuest software.
cMASCOT (∗) and SONAR (§) results (SwissProt & NCBInr databases).