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Schematic representation of the strategies used to generate random domain insertion libraries. (a) DNase I or S1 nuclease, in specific conditions, can generate a single break at the plasmid containing the acceptor gene. (b) Multiplex inverse PCR can open up the plasmid at targeted positions in the acceptor gene. (c) In vitro transposition uses an engineered transposon to randomly linearize the plasmid. The gene coding for the insert domain is ligated in all the approaches.
