Table 3.
Molecular identification of mosquitoes collected in Chad in October 2017 randomly selected for confirmation of MALDI-TOF MS identification
| Number of samples | Morphological identification | Log score value | MALDI-TOF MS identification | Molecular identification (COI gene) | Molecular identification (internal transcribed spacer 2 gene) |
|---|---|---|---|---|---|
| 632 | Anopheles sp. ♂ | 1.79 | / | Anopheles rhodesiensis/Anopheles rufipes* | An. rufipes |
| 595 | Anopheles sp. ♂ | 1.84 | An. rufipes | An. rufipes | An. rufipes |
| 657 | Anopheles sp. ♂ | 1.84 | An. rufipes | An. rufipes | – |
| 693 | Anopheles sp. ♂ | 1.854 | An. rufipes | An. rhodesiensis/An. rufipes* | An. rufipes |
| 599 | Anopheles sp. ♂ | 1.869 | An. rufipes | An. rufipes | – |
| 698 | Anopheles sp. ♂ | 1.879 | An. rufipes | An. rhodesiensis/An. rufipes* | An. rufipes |
| 621 | Anopheles sp. ♂ | 1.89 | An. rufipes | An. rufipes | – |
| 677 | Anopheles sp. ♂ | 1.91 | An. rufipes | An. rufipes | – |
| 587 | Anopheles sp. ♂ | 1.977 | An. rufipes | An. rufipes | – |
| 585 | Anopheles sp. ♂ | 2.005 | An. rufipes | An. rhodesiensis/An. rufipes* | – |
| 583 | Anopheles sp. ♂ | 2.023 | An. rufipes | An. rufipes | – |
| 563 | Anopheles sp. ♂ | 2.065 | An. rufipes | An. rufipes | – |
| 487 | Anopheles sp. ♂ | 2.11 | An. rufipes | An. rufipes | – |
| 491 | Anopheles gambiae ♀ | 2.126 | An. gambiae | An. gambiae | – |
| 593 | An. rufipes ♀ | 2.128 | An. rufipes | An. rufipes | – |
| 297 | An. gambiae ♀ | 2.23 | An. gambiae | An. gambiae | – |
| 572 | An. gambiae ♀ | 2.261 | An. gambiae | An. gambiae | – |
| 533 | Culex sp. ♀ | 1.037 | / | Culex watti | – |
| 694 | Culex sp. ♀ | 1.452 | / | Culex rima | – |
| 618 | Culex sp. ♀ | 1.513 | / | Culex perexiguus | – |
| 374 | Culex sp. ♀ | 1.635 | / | Cx. perexiguus | – |
| 556 | Culex sp. ♀ | 1.679 | / | Cx. perexiguus | – |
| 720 | Culex sp. ♀ | 1.73 | / | Cx. watti | – |
| 624 | Culex sp. ♀ | 1.956 | Culex pipiens | Cx. pipiens | – |
| 521 | Culex sp. ♂ | 2.131 | Culex quinquefasciatus | Cx. quinquefasciatus | – |
| 522 | Culex sp. ♂ | 2.139 | Cx. quinquefasciatus | Cx. quinquefasciatus | – |
| 740 | Culex sp. ♀ | 2.145 | Cx. pipiens | Cx. pipiens | – |
| 705 | Culex sp. ♀ | 2.161 | Cx. pipiens | Cx. pipiens | – |
| 715 | Culex sp. ♀ | 2.161 | Cx. pipiens | Cx. pipiens | – |
| 568 | Culex sp. ♀ | 2.253 | Cx. quinquefasciatus | Cx. quinquefasciatus | – |
| 682 | Culex sp. ♀ | 2.267 | Cx. quinquefasciatus | Cx. quinquefasciatus | – |
| 523 | Culex sp. ♂ | 2.314 | Cx. quinquefasciatus | Cx. quinquefasciatus | – |
| 520 | Culex sp. ♀ | 2.337 | Cx. pipiens | Cx. pipiens | – |
| 592 | Culex sp. ♀ | 2.427 | Cx. quinquefasciatus | Cx. quinquefasciatus | – |
MALDI-TOF MS = Matrix-assisted desorption/ionization time-of-flight mass spectrometry.
* Non-discriminative results (identical cover and identity values).