Figure 5.

Proliferation and viability of MSCs are not affected by nanoparticle labeling.

Notes: Cell viability assay was observed in MSCs labeled with different concentrations (0, 25, 50, 75, 100, 150, or 200 µg/mL) of nanoparticles for 24 hours. (A) Proliferation of 50 µg/mL nanoparticle-labeled MSCs was assessed after 5 days by CCK-8. (B) In vitro CFU assays are usually used to detect the proliferation and differentiation features of MSCs. A colony was defined as an aggregate of >40 cells. (C) CFU colonies of MSC-labeled nanoparticles (50 µg/mL) were stained with Giemsa stain, (D) and the number of colonies was counted.

Abbreviations: CCK-8, cell counting kit-8; CFU, colony-forming unit; Fe₃O₄@PDA, PDA-capped Fe₃O₄; PDA, polydopamine; MSC, mesenchymal stem cell.