FIG 4.

SNW1 is not a general transcription coactivator and modulates the NF-κB pathway in response to multiple stimuli. (A) THP-1 cells (with negative-control siRNA or SNW1 siRNA) transfected with constitutively active pTK Renilla luciferase show no significant change in constitutively active Renilla luciferase activity under siSNW1 conditions. (B) Knockdown of the NF-κB pathway (via sip65) increases TNF-α-mediated apoptosis in THP-1 cells. A similar result is seen when SNW1 is knocked down, providing evidence for SNW1’s role in the NF-κB pathway upon TNF-α treatment (P < 0.05 for both sip65 and siSNW1). (C) THP-1 cells (transduced with NF-κB luciferase) were transfected with a negative-control siRNA (si–ve), siSNW1, or sip65 and stimulated for 6 h with the above-mentioned stimuli. siRNA against SNW1 attenuates NF-κB-driven luciferase activity, while siRNA against p65 shows a similar effect with all stimuli (P < 0.05 for negative-control siRNA versus any condition for all stimulants tested).