FIG 6.

SNW1 is necessary for Pol II-dependent elongation of NF-κB target genes but not for the formation of the preinitiation complex. THP-1 cells under normal or siSNW1 conditions were treated with TNF-α for 60 min and immunoprecipitated for chromatin that complexed with either CDK9, p65, or Pol II. (A to F) Recruitment of CDK9 (A), p65 (B), and Pol II (C) on the IL-8 promoter is increased significantly upon TNF-α treatment, and the effect remains unchanged in cells that have SNW1 depleted. (A to C) The corresponding effects are similar for the TNF promoter. (D to H) The recruitment of Pol II on the coding sequence region of the IL-8 gene is increased upon TNF-α treatment in normal cells, but the effect is significantly reduced in SNW1-depleted cells (G), which is also observed for the coding sequence region of the TNF gene (H). Note that panels A to F have statistically insignificant differences (at an alpha value of 0.05) between the negative-control siRNA (TNF) and siSNW1 (TNF) columns, while in panels G and H, the corresponding differences are statistically significant.