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Validation of CDK9, p65, and Pol II antibodies for ChIP using a negative-control U6 snRNA sequence. In THP-1 cells, CDK9 (A), p65 (B), and Pol II (C) antibodies bind minimally to the Pol III-driven U6 snRNA sequence (as evidenced by the low percentage of the input compared to that in Fig. 6), and the binding is unaffected by TNF-α treatment or under SNW1 depletion conditions (statistically insignificant at an alpha value of 0.05) (n = 3 replicates).
