FIG 3.

Consequences of eIF1A depletion on several cell cycle-associated mRNAs. (A) Validation of eIF1A knockdown in the ribosome profile. Tracks showing the ribosome profiling reads along eIF1A (18) and eIF1AX mRNAs for replicate samples of each control and eIF1A KD. (B) Tracks showing the ribosome profiling reads along various cell cycle-associated mRNAs for replicate samples of each control and eIF1A KD. Blue bars, the actual translated coding sequence based upon ribosome profiling; red arrows, direction of translation. (C) Western blot analysis of genes from the cell cycle and DNA repair categories that were translationally downregulated upon eIF1A KD. The levels of eIF1A and GAPDH (a control for unaffected genes) are also shown.