FIG 6.

eIF1A is a central player in the eIF1 autoregulatory feedback loop. (A) Tracks showing the ribosome profiling reads along eIF1 mRNA for replicate samples of each control (green) and eIF1A KD (red). (B) eIF1 protein levels in control and eIF1A KD MEFs were analyzed by Western blotting along with those of eIF1A and GAPDH. This image is a representative of images from 3 independent experiments with similar results. (C) eIF1 mRNA levels in control and eIF1A KD MEFs derived from the RNA-seq data.