TABLE 3.
Primer list
| Primer name | Application | Sequence |
|---|---|---|
| GAPDH Forward | Real-time PCR | AGGTCGGTGTGAACGGATTTG |
| GAPDH Reverse | Real-time PCR | GGGGTCGTTGATGGCAACA |
| eIF1A (Chr18) Forward | Real-time PCR | GTTGCCCTTTCCATCATGCC |
| eIF1A (Chr18) Reverse | Real-time PCR | ACACCGTCAAAGCACATTGC |
| eIF1AX forward | Real-time PCR | GTGACCAGCCTCTCCTGAGC |
| eIF1AX Reverse | Real-time PCR | GGAACTGCGGTGACTCCGA |
| eIF1A G6D forward | Site-directed mutagenesis | CAAGAATAAAGATAAAGGAGGTAAAAAC |
| eIF1A G6D Reverse | Site-directed mutagenesis | GGCATTTAGATGTCATCAATATC |
| eIF1A G9D forward | Site-directed mutagenesis | GGTAAAGGAGATAAAAACAGAC |
| eIF1A G9D Reverse | Site-directed mutagenesis | TTTATTCTTGGGCATTTAG |
| eIF1A G9R forward | Site-directed mutagenesis | AGGTAAAGGACGTAAAAACAGACG |
| eIF1A G9R Reverse | Site-directed mutagenesis | TTATTCTTGGGCATTTAGATG |
| eIF1A G8R_G9R forward | Site-directed mutagenesis | TAAAGGTAAACGACGTAAAAACAGACGCAG |
| eIF1A G8R_G9R Reverse | Site-directed mutagenesis | TTCTTGGGCATTTAGATG |
| eIF1A G15D forward | Site-directed mutagenesis | CAGACGCAGGGATAAGAATGAGA |
| eIF1A G15D Reverse | Site-directed mutagenesis | TTTTTACCTCCTTTACCTTTATTCTTG |
| eIF1A K10E forward | Site-directed mutagenesis | TAAAGGAGGTGAAAACAGACGCAG |
| eIF1A K10E Reverse | Site-directed mutagenesis | CCTTTATTCTTGGGCATTTAG |
| eIF1A R13H forward | Site-directed mutagenesis | TAAAAACAGACACAGGGGTAAGAATG |
| eIF1A R13H Reverse | Site-directed mutagenesis | CCTCCTTTACCTTTATTCTTG |
| eIF1A Forward | PCR cloning | CATCACCATCATCACCACAGCCAGGATCCGATGCCCAAGAATAAAGGTA |
| eIF1A Reverse | PCR cloning | TCGACTTAAGCATTATGCGGCCGCAAGCTTTTAGATGTCATCAATATCTTCATC |
| Rps3 Forward | PCR cloning | ATGGCAGTGCAAATATCCAAG |
| Rps3 Reverse | PCR cloning | TCGACTTAAGCATTATGCGGCCGCAAGCTTTTATGCTGTGGGGACTGGCTGGGGCA |
| Rps10 Forward | PCR cloning | CATCACCATCATCACCACAGCCAGGATCCGATGTTGATGCCTAAGAAGAAC |
| Rps10 Reverse | PCR cloning | TCGACTTAAGCATTATGCGGCCGCAAGCTTTTACTGAGGTGGCTGACCACGTCC |