FIG 4.

Rab35-regulated TMV shedding requires contractile machinery. (A) LOX^Rab35KO cells were treated with the small molecule inhibitor ML-7 or vehicle control during amoeboid cell invasion. Scale bars, 25 µm. (B) The average displacement of cells described for panel A was calculated after 8 h of amoeboid invasion. Data are presented as means ± the SD (*, P < 0.001). (C) Nanoparticle tracking analysis of conditioned media from untreated, vehicle control, or ML-7-treated LOX^Rab35KO cells. Data are presented as means ± the SEM for each particle diameter. (D) LOX^Rab35KO cells were treated with the ROCK inhibitor Y-27632 or vehicle control during amoeboid cell invasion. Scale bars, 25 µm. (E) Nanoparticle tracking analysis of conditioned media from untreated, vehicle control, or Y-27632-treated LOX^Rab35KO cells. Data are presented as means ± the SEM at each particle diameter. (F) The average displacement of cells described for panel D was calculated after 8 h of amoeboid invasion. Data are presented as means ± the SD (*, P < 0.001). (G) The levels of phospho-AKT (Ser-473) were measured in LOX and LOX^Rab35KO cells by Western blotting. (H) Proliferation was quantified in LOX and LOX^Rab35KO cells using a sulforhodamine B assay. Data are presented as means ± the SD (*, P < 0.02).