Fig. 4. A. Bright field and confocal fluorescence images of the fixed SW480 cell incubated with DC473. The maps of each of the scores of the 4 main PCs are shown. B. Coefficients for the first 4 PCs whose scores are mapped in A. C. Bright field, fluorescence and score maps of PCA coefficients with DC473 associated Raman bands. The cell main features have been overlaid with the images as a visual aid. The presence of PC4 suggests that Raman could be detecting presence of DC473 in a region in the nucleus that does not show in the fluorescence image.
