Fig. 7. Mouse brain microvasculature reconstructed from 1000 KESM sections. (a) A raw section (x–y image) is shown, with microvascular cross-sections stained black using an India-ink perfusion. An inset shows a close-up of the India-ink embedded sample, in which vessels are stained black. (b) A maximum intensity projection of all 1000 KESM sections shows the overall structure of the sample. (c) An inset shows a viable ROI containing several inlets and outlets problematic for modeling. (d) Our approach represents the ROI network as a graph-like model with geometric components including points (orange dots), fibers (blue lines), and radii (dashed purple lines). The fiber lists F₀ and F₁ associated with each pivot point store fiber connectivity (e.g. a fiber in F₀ has geometry specified outward from the given point). An inset shows visualizing fibers as a series of truncated generalized cones (TGCs) in AFAN. (b) SB = 0.5 mm. (c) SB = 15 μm.