Figure 5. Statins induce transcription of PUMA and HRK in DLBCL and AML cell lines.
A-B, western blot (left) and quantification (right) of whole cell lysates from DLBCL cells (A) or AML cells (B) treated with 10 µM of indicated inhibitors for 16 hours. C, western blot (left) and quantification (right) of mitochondria-enriched fractions from DLBCL cells treated with 10 µM of indicated inhibitors for 16 hours, M = mitochondrial and C = cytoplasmic fractions. COX IV is a mitochondrial marker, and ERK is a cytoplasmic marker. D, qPCR of DLBCL cell lines treated with 10 µM of indicated inhibitors for 16 hours. (A-C) Paired one-tailed t-tests, n = 3–5. (D) One-way ANOVA with Dunnett’s post-test (all samples compared to control).