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. 2019 Jan;60(1):245–254. doi: 10.1167/iovs.18-24345

Figure 5.

Figure 5

Supernatants from HCLE cells previously incubated with anti-EMP2 antibody show reduction in secreted VEGF and reduced cell migration in a transwell migration assay. (A) Secreted VEGF-A was measured by ELISA in the cell culture supernatants of HCLE cells incubated with anti-EMP2 antibody for 0 to 48 hours. There is a time-dependent reduction in VEGF secretion with EMP2 blockade. (B) Supernatants of HCLE cells incubated with anti-EMP2 for 0 to 48 hours were used to test function in an HUVEC transwell migration assay. Of note, there is a statistically significant reduction in the number of cells that have migrated after 12 and 24 hours (*P < 0.05).