Fig 5. Anti-SVCV infectivity after treatment of EPC cell monolayers with 25HOCh and CRP1-7.

A) EPC cell monolayers were incubated with 100 μl of ssGFP or ssCRP1-7 4-fold diluted in RPMI with 2% FBS ± 10 μM 25HOCh for 20 h at 26°C. After washing, 10⁻² m.o.i of SVCV were added and incubated for 24 h. After staining with anti-SVCV and fluorescein-labeled goat anti-mouse immunoglobulins [7], the number of fluorescent cells were estimated by flow cytometry. B) Representative aspects of histograms from nonfluorescent and fluorescent cells. The number of SVCV-infected EPC cells varied from 12.7 to 50.6% (n = 5), depending on the experiment. The results were expressed as relative infection percentages calculated by the following formula, 100 × (number of infected cells+25HOCh / number of infected cells -25HOCh). The means and standard deviations of a representative experiment were represented (n = 3). *, statistically < than cells transfected with ssGFP at p < 0.05 (Student's t-test).