Skip to main content
. 2019 Jan 17;17(1):e2006749. doi: 10.1371/journal.pbio.2006749

Fig 3. 21G11-LexA neurons are presynaptic to 23C09∩VGlut neurons.

Fig 3

(A) LH showing GRASP, a measure of contact between the membranes of neurons, in 21G11-LexA and 23C09∩VGlut. (B) Schematics of the experiment and calcium response at the LH, using GCaMP6m, of 23C09∩VGlut neurons to air, 0.5%, 1%, and 2% of CO2 while 21G11-LexA neurons are silenced by expression of Kir2.1. (C) Peak GCaMP6m intensity change after stimulation with air, 0.5%, 1%, and 2% of CO2. (D) Peak GCaMP6m intensity change between Fig 2F and Fig 3C after stimulation with air, 0.5%, 1%, and 2% of CO2. (E) Schematics of the experiment and calcium response at the LH of 23C09∩VGlut during baseline, activity without retinal, and upon activation with 720 nm light of 21G11-LexA driving expression of Chrimson. (F) Peak GCaMP6m intensity change of 23C09∩VGlut during baseline, activity without retinal, and upon activation with 720 nm light of 21G11-LexA driving expression of Chrimson. (G) Schematics of the experiment and calcium response at the LH, using GCaMP6m, of 21G11-LexA neurons to air, 0.5%, 1%, and 2% of CO2 while 23C09∩VGlut neurons are silenced by expression of Kir2.1. (H) Peak GCaMP6m intensity change after stimulation with air, 0.5%, 1%, and 2% of CO2. (I) Schematics of the experiment and LH activity of 21G11-LexA upon activation of 23C09∩VGlut neurons, expressing Chrimson, with 720 nm light. (J) Peak GCaMP6m intensity change of 21G11-LexA during baseline and activation with 720 nm light of 23C09∩VGlut driving expression of Chrimson. (K) Proposed model of the LHNs processing CO2 information. Elements in dashed lines are hypothetical. For (B), (E), (G), and (I), the average time course of GCaMP6m intensity change is shown. The black bar indicates the time of the stimulus. Scale bar = 10 μm. *p < 0.05, **p < 0.01, ****p < 0.0001. All p values are calculated with Wilcoxon signed-rank test. d, dorsal; GFP, green fluorescent protein; GRASP, GFP reconstitution across synaptic partners; l, lateral; LH, lateral horn; LHN, LH neuron; LHON, LH output neuron; m, medial; SIP, superior intermediate protocerebrum; v, ventral.