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. 2019 Jan 17;17(1):e2006571. doi: 10.1371/journal.pbio.2006571

Fig 6. Oct3 deficiency induced a thermogenesis and lipolysis program through the NE/β-AR/PKA signaling pathway.

Fig 6

(A–C) Maximal body temperature change (panel A), maximal O2 consumption change (ΔO2) (panel B), and maximal heat production change (ΔHeat) (panel C) of Ctrl and cKO mice (n = 6 in body temperature measurement; n = 4 for oxygen consumption and heat production measurement). NE was injected into mice with propranolol (“NE + prop”) or without propranolol (5 mg/kg, s.c.) pretreatment (“NE + vehicle”). (D) The OCR in Oct3 KO beige adipocytes and Ctrl. Cells were acutely stimulated with NE in the absence or presence of propranolol at the indicated time point (arrow). Ctrl or Oct3 KO adipocytes with NE (n = 7 for both); Ctrl or Oct3 KO adipocytes with NE and propranolol (n = 8 for both). (E) Ucp1 mRNA expression in differentiated primary inguinal adipocytes from Ctrl and cKO mice after stimulation with NE and T3 in the presence or absence of propranolol (n = 3). (F) Western blotting of pPKA substrate, pCreb, and total Creb in primary inguinal adipocytes from Ctrl and cKO mice stimulated with NE in the presence or absence of propranolol. (G) In vitro glycerol release from differentiated SVF-derived adipocytes in the presence or absence of NE and propranolol (n = 3). (H) Western blotting of pHsl-S563, pHsl-S660, and total Hsl in differentiated SVF-derived adipocytes from Ctrl and cKO mice in the presence or absence of NE and propranolol. (I) Schematic diagram of Oct3-regulating thermogenesis and lipolysis in ingWAT. Data in A–E and G were analyzed by one-way ANOVA followed by Tukey’s test. Data in I and K were analyzed by Student t test. The numerical data underlying this figure are included in S1 Data. β-AR, β-adrenergic receptor; AC, adenylyl cyclase; cAMP, cyclic adenosine monophosphate; Creb, cAMP-responsive element binding protein; Ctrl, control; FFA, free fatty acid; Gs, Gs alpha subunit; Hsl, hormone-sensitive lipase; ingWAT, inguinal white adipose tissue; KO, knockout; NE, norepinephrine; OCR, O2 consumption rate; Oct3, organic cation transporter 3; pCreb, phosphorylated cAMP-responsive element binding protein; pHsl, phosphorylated hormone-sensitive lipase; PKA, protein kinase A; pPKA, phosph-PKA; prop, propranolol; s.c., subcutaneous; SVF, stromal vascular fraction; T3, triiodothyronine; TF, transcription factor; TG, triglyceride; VO2, oxygen consumption.