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. 2019 Jan 17;14(1):e0210243. doi: 10.1371/journal.pone.0210243

Fig 1. Design and application of cDNA006 for disruption of albA.

Fig 1

(A) of cDNA006 contains pyrG flanked by 300bp repeats at 1000 bp homology arms to albA. After integration, pyrG is excised by homologous recombination in the presence of 5-FOA. (B) and (C) Phenotypes obtained before 5-FOA and after 5-FOA. (D) Representative sequence showing the integration of the stop codon at the albA locus in a white colony.