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(A) cDNA009 construct inserted at the glaA locus to insert A5IL97 gene making A. niger pyrG− ΔglaA/PglaA-A5IL97, use of sgRNA002 and sgRNA003. (B) Design of cDNA010 construct inserted at the mstC locus to delete mstC on the A. niger pyrG− ΔglaA/PglaA-A5IL97, resulting in the strain A. niger pyrG− ΔglaA/PglaA-A5IL97 ΔmstC.
