Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 17;14(1):e0209995. doi: 10.1371/journal.pone.0209995

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Huang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 3 — (A, B, and C) Spermatogonia from DCUN1D1^-/- mice have normal proliferative activity. Representative results from immunostaining of testis sections, showing similar patterns of BrdU (A), Ki-67 (B), and phospho-histone H3 (C) expression in spermatogonia from DCUN1D1^-/- and DCUN1D1^+/+ mice, which suggests that mitotic activity is intact (scale bar, 50 μm). (D) Sertoli cell numbers are normal in DCUN1D1^-/- mice. Immunostaining for p27, which is exclusively expressed in Sertoli cells, confirmed that Sertoli cells were present in adequate number in DCUN1D1^-/- mice (23.5±8.0 per section, n = 8), compared with DCUN1D1^+/+ mice (25.5±8.5 per section, n = 6) (scale bar, 50 μm). (E) Results of TUNEL assays on testis sections from DCUN1D1^+/+ (left panels), DCUN1D1^+/- (middle panels), and DCUN1D1^-/- (right panels) mice at 6 weeks (top panels) and 3 months (bottom panels) of age. Apoptosis was increased only in testis from 3-month-old DCUN1D1^-/- mice (scale bar, 100 μm).