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. 2019 Jan 17;14(1):e0209995. doi: 10.1371/journal.pone.0209995

Fig 6. Defect of spermiation in DCUN1D1-/- mice.

Fig 6

Testis sections from DCUN1D1+/+ and DCUN1D1-/- mice at 6 weeks old were stained with DAPI and staged. An increase in the intensity of the DAPI signal is consistent with progressive condensation of nuclei during spermatid development (see the spermatids in the luminal aspect of the tubules). Testes from DCUN1D1+/+ mice show normal release of mature spermatozoa into the lumen of seminiferous tubules starting from stage 8. In contrast, the majority of spermatozoa with condensed nuclei in DCUN1D1-/- mice are not released, resulting in the presence of two generations of developing spermatids coexisting in a single tubule (stage 9 to 12).