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. 2019 Jan 17;14(1):e0210513. doi: 10.1371/journal.pone.0210513

Fig 9. PG treatment causes changes in autophagy- and apoptosis-related proteins.

Fig 9

HepJ5 or Mahlavu were treated with 80 ug/ml PG or vehicle for 24 h. A. The levels of autophagy related proteins (ATG5, ATG12, Beclin-1, LC3) were checked by western blotting. The amount of ATG5, ATG5+12, becklin-1 was similar between PG-treated and vehicle-treated sample. The ratio of LC3-II/LC3-I was high in PG treated sample. B. The cell apoptosis related proteins, Bcl-2, Bax, Bad, c-PARP, and c-caspase3 was checked. PG treated cells showed decreased Bcl-2 (anti-apoptotic), increased Bax and Bad protein (pro-apoptotic) and increased cleavage PARP (C-PARP) and cleavage caspase 3 (C-caspase3). All experiments were repeated at least three times independently.