A)
C. albicans flo8Δ/flo8Δ and mfg1Δ/mfg1Δ mutants are largely blocked in filamentation in response to most cues, except in response to Hsp90 inhibition by treatment with geldanamycin (GdA). In contrast, a C. albicans mss11Δ/mss11Δ mutant was able to filament in response to all cues tested. Cells were grown in the conditions indicated, and were imaged after 3.5 hours, except for those grown in the presence of GdA, which were imaged after 24 hours. Scale bar is 20 μm. B)
FLO8 is required for filamentation induced by deletion of the negative regulators of filamentation, NRG1 or LRG1, while MFG1 is not required. Cells were grown in YPD for 5 hours at 30°C. Scale bar is 20 μm.