Fig 3. C. albicans Mfg1 partially complements the activity of its S. cerevisiae ortholog.

A) C. albicans FLO8 or MFG1 were expressed in diploid cells of the filamentation-competent S. cerevisiae Σ1278b strain (Sc Wild type) and in the S. cerevisiae strains lacking the relevant orthologs to assess the ability of these complex members to complement the block in S. cerevisiae diploid pseudohyphal growth. Cells were grown on SLAD agar plates and incubated at 30°C for 11 days. Scale bar is 1.0 mm. B) C. albicans FLO8 or MFG1 were expressed in S. cerevisiae Σ1278b haploid cells (Sc Wild type) to assess their abilities to complement lack of FLO8 or MFG1 in S. cerevisiae haploid invasive growth. Cells were spotted onto YPD agar plates and incubated at 30°C for 4 days at which point they were washed with water and images of the plate were taken with a Canon Power Shot A610. Scale bar is 1.0 cm.