Fig 5. ChIP-chip and transcriptional analyses reveal targets of Mfg1 and Flo8, and AP-MS reveals continued physical interaction under filament-inducing conditions.

A) Mfg1 and Flo8 bind and regulate overlapping and distinct targets, which are condition specific. Venn diagrams indicate number of genes whose promoters were bound by Mfg1 or Flo8 in filament-inducing conditions (either 1 hour or 3 hours), and for which expression was altered in the respective null mutants at the respective time point. Select targets in serum-treated conditions that are related to filamentation are indicated below the relevant category. B) AP-MS was performed with Mfg1-GFP cells grown in untreated conditions, or in the presence of serum at 37°C for 1 hour or 3 hours. Shown are prey proteins with a minimum of 25 detected peptides averaged between two biological replicates, in at least one of the three growth conditions. Mfg1, Flo8, and Mss11 are indicated in red. The separate Nop5 entries correspond to unique BioGrid IDs. Inside circle colour indicates average spectral counts, size of the circle indicates relative protein abundance, and the rim of the circle indicates the Bayesian false discovery rate (BFDR).