Figure 1. Illustration of host-symbiont pairs used throughout the study.

D. discoideum clones were originally harvested from the wild in three different states: uninfected (indicated as naïve), or naturally infected with B. agricolaris or B. hayleyalla (indicated as native-ag, and native-ha respectively). Clones were treated with antibiotics to eliminate symbionts and are indicated with a ‘.c’. Clones were subsequently exposed to Burkholderia to initiate new infections. Thus, experimental types include 1) Field harvested, 2) cured, and 3) lab infected hosts.