Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapiens) |
LNCaP | ATCC | CRL-1740, RRID:CVCL_1379 |
|
| Cell line (H. sapiens) |
LNCaP/AR | PMID: 14702632 | ||
| Cell line (H. sapiens) |
CWR22Pc-EP | PMID: 28059768 | ||
| Antibody | AR | abcam | ab108341, RRID:AB_10865716 |
WB (1:1000), IP (5 ug/IP) |
| Antibody | AR | Santa Cruz | sc-816, RRID:AB_1563391 |
IF (1:500), ChIP (5 ug/IP) |
| Antibody | AR | Agilent | 441 | IHC |
| Antibody | KLK3 | Cell Signaling Technology |
5365 | WB (1:500) |
| Antibody | KLK3 | Biogenex | IHC | |
| Antibody | FKBP5 | Cell Signaling Technology |
8245, RRID:AB_10831198 |
WB (1:500) |
| Antibody | TRPM8 | Epitomics | 3466–1, RRID:AB_10715643 |
WB (1:1000) |
| Antibody | tubulin | Santa Cruz | sc-9104, RRID:AB_2241191 |
WB (1:1000) |
| Antibody | Cyclophilin B | abcam | ab178397 | WB (1:100,000) |
| Antibody | BRD4 | Cell Signaling Technology |
13440, RRID:AB_2687578 |
WB (1:1000) |
| Antibody | TOP2B | abcam | ab58442, RRID:AB_883147 |
WB (1:1000) |
| Antibody | HA | Cell Signaling | 3724, RRID:AB_1549585 |
WB (1:1000) |
| Antibody | HA | Abcam | ab9110, RRID:AB_307019 |
ChIP (5 ug/IP) |
| Antibody | Alexa Fluor 647 | Invitrogen/ Thermo Fisher |
A-31573, RRID:AB_2536183 |
IF (1:1000) |
| Antibody | p300 | Santa Cruz | sc-585, RRID:AB_2231120 |
ChIP (5 ug/IP) |
| Antibody | ER | Santa Cruz | sc-8002, RRID:AB_627558 |
ChIP (5 ug/IP) |
| Antibody | normal rabbit IgG | Millipore Sigma | 12–370, RRID:AB_145841 |
ChIP (5 ug/IP) |
| Antibody | Protein A/G agarose beads |
Santa Cruz | sc-2003, RRID:AB_10201400 |
|
| Recombinant DNA reagent |
ARR3tk-eGFP/ SV40-mCherry |
This paper | Addgene plasmid #24304 |
|
| Recombinant DNA reagent |
SCEP-shRenilla | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shAR.177 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shGREB1-1 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shGREB1-2 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shGREB1-3 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shKLF8.3467 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shKLF8.2180 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shKLF8.2684 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shGHRHR.544 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shGHRHR.1571 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shGHRHR.1583 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-sh-p300-1 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-sh-p300-2 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shSRC1-1 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shSRC2-1 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
SCEP-shSRC2-2 | This paper, PMID: 24332856 |
||
| Recombinant DNA reagent |
lentiCRISPRv2-SgNT | PMID: 24336569 | Addgene plasmid #52961 |
|
| Recombinant DNA reagent |
lentiCRISPRv2- SgGREB1-7 |
This paper | Addgene plasmid #52961 |
|
| Recombinant DNA reagent |
lentiCRISPRv2- SgGREB1-8 |
This paper | Addgene plasmid #52961 |
|
| Recombinant DNA reagent |
pCMV6-GREB1 | PMID: 23403292 | ||
| Recombinant DNA reagent |
pLVX-TRE3G- HA-GREB1 |
This paper | ||
| Sequence- based reagent |
q-PCR primers | This paper | See Supplementary file 1 |
|
| Sequence- based reagent |
shRNAs | This paper | ||
| Sequence- based reagent |
gRNAs | This paper | ||
| Commercial assay or kit |
QIAshredder | Qiagen | 79656 | |
| Commercial assay or kit |
RNeasy Mini Kit | Qiagen | 74106 | |
| Commercial assay or kit |
High Capacity cDNA Reverse Transcription Kit |
thermo fisher | 4368814 | |
| Commercial assay or kit |
QuantiFast SYBR Green PCR Kit |
Qiagen | 204057 | |
| Commercial assay or kit |
BCA Protein Assay | ThermoFisher | 23225 | |
| Commercial assay or kit |
Subcellular Protein Fractionation Kit |
ThermoFisher | 78840 | |
| Commercial assay or kit |
Peira TM900 system | Peira bvba | ||
| Commercial assay or kit |
the KAPA Biosystems Hyper Library Prep Kit |
Kapa Biosystems | KK8504 | |
| Chemical compound, drug |
FBS | Omega Scientific | FB-11 | |
| Chemical compound, drug |
Accumax | Innovative Cell Technologies |
AM105 | |
| Chemical compound, drug |
matrigel | Corning | 356237 | |
| Chemical compound, drug |
Laemmli sample buffer |
BioRad | 1610747 | |
| Chemical compound, drug |
4% formaldehyde | electron microscopy sciences |
15714 s | |
| Chemical compound, drug |
normal goat serum | Vector Lab | S-1000, RRID:AB_2336615 |
|
| Chemical compound, drug |
normal horse serum | Vector Lab | S-2000, RRID:AB_2336617 |
|
| Chemical compound, drug |
10% Triton X- 100 solution |
Teknova | T1105 | |
| Chemical compound, drug |
DAPI mounting solution |
Vector Lab | H-1200, RRID:AB_2336790 |
|
| Chemical compound, drug |
charcoal-stripped dextran-treated fetal bovine serum |
Omega Scientific | FB-04 | |
| Chemical compound, drug |
Puromycin | Invivogen | ant-pr | |
| Chemical compound, drug |
RPMI | Media Preparation Core at Sloan Kettering Institute |
||
| Chemical compound, drug |
DMEM | Media Preparation Core at Sloan Kettering Institute |
||
| Software, algorithm |
Partek Genomics Suite software |
Partek Inc | RRID:SCR_011860 | |
| Software, algorithm |
FlowJo software | FlowJo software | RRID:SCR_008520 | version 9.9.6 |
| Software, algorithm |
GSEA | Broad Institute | RRID:SCR_003199 | http://www.broadinstitute.org/gsea/index.jsp |
| Software, algorithm |
GraphPad Prism | GraphPad Prism | RRID:SCR_002798 | version 7 |
| Software, algorithm |
STAR aligner | PMID: 23104886 | RRID:SCR_015899 | |
| Software, algorithm |
Kalisto | PMID: 27043002 | ||
| Software, algorithm |
RSeQC | PMID: 22743226 | RRID:SCR_005275 | http://broadinstitute.github.io/picard/ |
| Software, algorithm |
DESeq2 package | http://www-huber.embl.de/users/anders/DESeq | RRID:SCR_015687 | |
| Software, algorithm |
Picard | http://broadinstitute.github.io/picard/index.html | RRID:SCR_006525 | |
| Software, algorithm |
MACS2 | PMID: 22936215 | ||
| Software, algorithm |
ChAsE | PMID: 27378294 | ||
| Software, algorithm |
MEME-ChIP | PMID: 21486936 | ||
| Software, algorithm |
HOMER | http://homer.ucsd.edu/homer/ | RRID:SCR_010881 |