FIG 3.

Expression of raffinose pathway genes by serotype 14 and 3 blood and ear isolates. The indicated strains were grown in CDM+Glc to an OD₆₀₀ of 0.2, washed and resuspended in CDM+Raf, and then incubated at 37°C for a further 30 min. RNA was then extracted, and levels of aga, rafG, and rafK mRNA were analyzed by qRT-PCR using 16S rRNA as an internal control (see Materials and Methods). The data presented are the means ± SD from three independent experiments. *, P < 0.05, **, P < 0.01, and ****, P < 0.0001, by unpaired t test.