TABLE 4.
Oligonucleotide primers used in this study
| Primer | Sequence (5′→3′) | Reference |
|---|---|---|
| rafR Flank F | GCGAACGTAGGTTACAATCGT | This study |
| rafR R j tail | GGAAAGGGGCCCAGGTCTCTCTAGCATGTGCTACCTCCTACC | This study |
| rafR F j tail | CATTATCCATTAAAAATCAAAGGGGAAATCCTACCAAGCTGTCTACC | This study |
| rafR Flank R | CGAACGTAGTTCAGTGGTAGAA | This study |
| Janus F | CCGTTTGATTTTTAATGGATAATG | 33 |
| Janus R | AGAGACCTGGGCCCCTTTCC | 33 |
| aga F | AAGGTCAGAATGGTCCACAG | This study |
| aga R | GCTGGAAAATCAGCCATAAA | This study |
| rafG F | CCTATGGCAGCCTACTCCATC | This study |
| rafG R | GGGTCTGTGGAATCGCATAGG | This study |
| rafK F | AACGACGTAGCTCCAAAAGA | This study |
| rafK R | GCTGGTTTACGTTCCAAGAA | This study |
| 16s rRNA F | GGTGAGTAACGCGTAGGTAA | 34 |
| 16s rRNA R | ACGATCCGAAAACCTTCTTC | 34 |
| rafR sanger | AGTAGAAGAGCTGGTGTTTG | This study |
| rafR sanger | TCTGTGACTAAGCCAGTTTC | This study |
| rafK Flank F | AGGACTTGGTTCTTGTTGAG | This study |
| rafK R ery tail | TTGTTCATGTAATCACTCCTTCCTACCATGAGGTGAACTCC | This study |
| rafK F ery tail | CGGGAGGAAATAATTCTATGAGATCAGTTAATCTAGGGAGAG | This study |
| rafK Flank R | CTCAAAGGCAACTGGACAAC | This study |