TABLE 1.
Mutations found in E. coli MG1655 (lac plus) after 1,000 generations of growth under LSMMG conditions with background exposure to chloramphenicola
| Position | Type of change | Mutation type(s) | Annotation | Gene(s) | Usual product |
|---|---|---|---|---|---|
| 151656 | T→G | Base change | A192A (GCA→GCC) | yadL ← | Predicted fimbrial-like adhesin protein |
| 483212 | A→G | Base change | V139A (GTT→GCT) | acrB ← | Multidrug efflux system protein |
| 883682 | C→T | Base change | P263S (CCT→TCT) | mdfA → | Multidrug efflux system protein |
| 4187022 | C→G | Base change | P1217R (CCG→CGG) | rpoC → | RNA polymerase, beta prime subunit |
| 4463866 | A→T | Base change | V113E (GTG→GAG) | treB ← | Fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component |
| 4540294 | G→A | Base change | E79K (GAG→AAG) | fimE → | Tyrosine recombinase/inversion of on/off regulator of fimA |
| 1617535 | A→T | Base change | E131V (GAA→GTA) | marR → | DNA-binding transcriptional repressor of multiple antibiotic resistance |
| 1816409 | A→C | Base change | V39G (GTG→GGG) | chbF ← | Phosphochitobiase; general 6-phospho-beta-glucosidase activity |
| 250390 | A→G | Base change | Pseudogene (319/756 nt) |
lafU → | Pseudogene, lateral flagellar motor protein fragment |
| 547694 | A→G | Base change | Pseudogene (114/252 nt) |
ylbE → | Predicted protein, C-ter fragment (pseudogene) |
| 547835 | +G | Base insertion/ addition |
Pseudogene (4/1,008 nt) |
ylbE → | Predicted protein, C-ter fragment (pseudogene) |
| 484938 | A→G | Base change | Intergenic (95/47) | acrA ← / → acrR | Multidrug efflux system/DNA-binding transcriptional repressor |
| 882870 | G→A | Base change | Intergenic (259/26) | ybjG ← / → mdfA | Undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein |
| 3826853 | T→C | Base change | Intergenic (165/115) | gltS ← / → xanP | Glutamate transporter/xanthine permease |
| 4541135 | A→C | Base change | Intergenic (+479/3) | fimE → / → fimA | Tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
| 986125 | Δ203 bp | Base deletion(s) | Coding | ompF ← | Outer membrane porin |
| 3558478 | Δ1 bp | Base deletion | Coding (151/759 nt) | glpR ← | DNA-binding transcriptional repressor |
| 257900 | IS1 (–) + 8 bp | TMR | Coding (7279/402 nt) | crl → | Sigma factor-binding protein (stimulates RNA polymerase holoenzyme formation) |
| 882777 | IS30 (+) + 2 bp | TMR | Intergenic (166/118) | ybjG ← / → mdfA | Undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein |
| 1298718 | IS5 (+) + 4 bp | TMR | Intergenic (+250/485) | ychE → / → oppA | Predicted inner membrane protein/oligopeptide transporter subunit |
| 1871055 | IS1 (–) + 9 bp | TMR | Coding (991999/ 1,491 nt) |
yeaJ → | Predicted diguanylate cyclase |
| 1962213 | Δ14,314 bp | TMR | IS1 mediated | (flhAB cheZYBR tap tarc cheWA motBA flhCD) ← |
Chemotaxis, flagellum, motility proteins |
a
←, gene orientation on reverse strand; →, gene orientation on positive strand; ←/→, intergenic; Δ, deletion; C-ter, C-terminal; IS, insertion sequence; PTS, phosphotransferase system; TMR, transposon-mediated rearrangement.