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. Author manuscript; available in PMC: 2019 Jan 17.
Published in final edited form as: J Am Chem Soc. 2017 Nov 10;139(46):16932–16938. doi: 10.1021/jacs.7b09932

Figure 1.

Figure 1

Ensemble and CDMS measurements provide a consistent view of capsid assembly. (A) Plot of the intensity of the light scattered by the capsid against time for the assembly of 5 µM dimer in ammonium acetate concentrations ranging from 200 mM to 1M. (B) Size exclusion chromatography (SEC) measurements for the assembly of 5 to 25 µM dimer in 210 mM ammonium acetate. The SEC measurements were performed 24 h after initiation of the assembly reaction. Dimer assigned to the free dimer pool (red squares) and dimer in capsid (blue circles) are plotted against the total dimer concentration. The lines show an equilibrium-based fit used to determine the pseudocritical concentration. (C) CDMS spectrum measured 24 h after initiating assembly with a dimer concentration of 5 µM in 210 mM ammonium acetate. The spectrum was generated by sorting the masses into 20 kDa bins. The inset shows an expanded view of the low mass region generated with 5 kDa bins.